anti hbb Search Results


hbb  (Proteintech)
93
Proteintech hbb
Hematopoietic progenitor cell formation in induced pluripotent stem (iPS)–hematopoietic stem‐progenitor cell cultures is dependent on stage‐specific notch signaling. (A): Schematic diagram illustrating gamma secretase inhibitor (GSI) dosing scheme. (B): Photomicrograph of D15 cell cultures. Cultures were treated with GSI at D4–5 or D4 onward (D4+) and display dramatically reduced hematopoietic cell emergence. (C): Quantification of D15 suspension hematopoietic cells generated in control/GSI‐treated cultures; cell numbers were normalized to the control for each experimental set. Error bars = SD. Statistical significance determined by Student's t test analysis. (D): Analysis of D10 control and GSI‐treated cultures for CD34 and CD45 revealed intact endothelial cell formation (CD34 + CD45 ‐ ) but reduced hematopoietic cell formation (CD34 + CD45 + ) in GSI‐treated cultures. (E): Gene expression analyses of D10 control versus GSI‐treated cultures for a panel of hematopoietic and signaling genes of interest. DLL4, HEY1, GATA2, and AML1 are significantly downregulated in GSI‐treated cultures, as determined by Student's t test analysis. All expression levels normalized to beta actin housekeeping gene. Error bars = SD. (F): Globin gene expression analyses of erythroid cells generated from control and stage‐specific GSI‐treated cultures. Error bars = SD. Statistical significance determined by Student's t test analysis. Abbreviations: EHT, endothelial‐to‐hematopoietic transition; GATA2; GSI, gamma secretase <t>inhibitor;</t> <t>HBA,</t> hemoglobin comprised of alpha; <t>HBB,</t> hemoglobin comprised of beta; HBE, embryonic hemoglobin; HBG, hemoglobin comprised of gamma; HSPC, hematopoietic stem‐progenitor cell; iPSC, induced pluripotent stem cell; RUNX1; SCL.
Hbb, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/HBB+Antibody/pmc06099224-79-5-7
Average 93 stars, based on 1 article reviews
hbb - by Bioz Stars, 2026-09
93/100 stars
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hbb antibody  (Aviva Systems)
86
Aviva Systems hbb antibody
Hematopoietic progenitor cell formation in induced pluripotent stem (iPS)–hematopoietic stem‐progenitor cell cultures is dependent on stage‐specific notch signaling. (A): Schematic diagram illustrating gamma secretase inhibitor (GSI) dosing scheme. (B): Photomicrograph of D15 cell cultures. Cultures were treated with GSI at D4–5 or D4 onward (D4+) and display dramatically reduced hematopoietic cell emergence. (C): Quantification of D15 suspension hematopoietic cells generated in control/GSI‐treated cultures; cell numbers were normalized to the control for each experimental set. Error bars = SD. Statistical significance determined by Student's t test analysis. (D): Analysis of D10 control and GSI‐treated cultures for CD34 and CD45 revealed intact endothelial cell formation (CD34 + CD45 ‐ ) but reduced hematopoietic cell formation (CD34 + CD45 + ) in GSI‐treated cultures. (E): Gene expression analyses of D10 control versus GSI‐treated cultures for a panel of hematopoietic and signaling genes of interest. DLL4, HEY1, GATA2, and AML1 are significantly downregulated in GSI‐treated cultures, as determined by Student's t test analysis. All expression levels normalized to beta actin housekeeping gene. Error bars = SD. (F): Globin gene expression analyses of erythroid cells generated from control and stage‐specific GSI‐treated cultures. Error bars = SD. Statistical significance determined by Student's t test analysis. Abbreviations: EHT, endothelial‐to‐hematopoietic transition; GATA2; GSI, gamma secretase <t>inhibitor;</t> <t>HBA,</t> hemoglobin comprised of alpha; <t>HBB,</t> hemoglobin comprised of beta; HBE, embryonic hemoglobin; HBG, hemoglobin comprised of gamma; HSPC, hematopoietic stem‐progenitor cell; iPSC, induced pluripotent stem cell; RUNX1; SCL.
Hbb Antibody, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/HBB+Antibody+(OALA04060)/pmc04851882-161-22-24
Average 86 stars, based on 1 article reviews
hbb antibody - by Bioz Stars, 2026-09
86/100 stars
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βa globin chain  (Rockland Immunochemicals)
93
Rockland Immunochemicals βa globin chain
The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). <t>a</t> UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of <t>IL1</t> <t>β</t> and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.
βa Globin Chain, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/Hemoglobin+A+(beta+chain)+Antibody/pmc11961595-318-33-30
Average 93 stars, based on 1 article reviews
βa globin chain - by Bioz Stars, 2026-09
93/100 stars
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anti-hbb  (Abnova)
90
Abnova anti-hbb
The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). <t>a</t> UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of <t>IL1</t> <t>β</t> and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.
Anti Hbb, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/anti+hbb/bio_rxiv__2022__07__19__500441-36-52-54
Average 90 stars, based on 1 article reviews
anti-hbb - by Bioz Stars, 2026-09
90/100 stars
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anti-hbb-y  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company anti-hbb-y
The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). <t>a</t> UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of <t>IL1</t> <t>β</t> and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.
Anti Hbb Y, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/anti+hbb+y/pmc11467802-88-29-30
Average 90 stars, based on 1 article reviews
anti-hbb-y - by Bioz Stars, 2026-09
90/100 stars
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antibody anti-hbb  (Abnova)
90
Abnova antibody anti-hbb
The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). <t>a</t> UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of <t>IL1</t> <t>β</t> and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.
Antibody Anti Hbb, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/antibody+anti+hbb/pmc10585431-44-14-16
Average 90 stars, based on 1 article reviews
antibody anti-hbb - by Bioz Stars, 2026-09
90/100 stars
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native 13c-hbb 13c-dps (syn nd anti isomers  (Cambridge Isotope Laboratories)
90
Cambridge Isotope Laboratories native 13c-hbb 13c-dps (syn nd anti isomers
The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). <t>a</t> UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of <t>IL1</t> <t>β</t> and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.
Native 13c Hbb 13c Dps (Syn Nd Anti Isomers, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/native+13c+hbb+13c+dps++syn+nd+anti+isomers/pm23992842-34-23-41
Average 90 stars, based on 1 article reviews
native 13c-hbb 13c-dps (syn nd anti isomers - by Bioz Stars, 2026-09
90/100 stars
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hbb  (Sangon Biotech)
86
Sangon Biotech hbb
The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). <t>a</t> UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of <t>IL1</t> <t>β</t> and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.
Hbb, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hbb/anti+hbb/pm39700741-111-8-11
Average 86 stars, based on 1 article reviews
hbb - by Bioz Stars, 2026-09
86/100 stars
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rabbit anti-mouse hbb-b2 polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Mouse Hbb-b2 Polyclonal Antibody
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rabbit anti-human hbb polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human HBB Polyclonal Antibody
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anti-hbb antibody  (St Johns Laboratory)
N/A
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta
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anti-hbb monoclonal antibody, clone 8c23  (Creative BioMart)
N/A
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.http://www.creative-diagnostics.com/HBB-antibody-60425-144.htm
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Image Search Results


Hematopoietic progenitor cell formation in induced pluripotent stem (iPS)–hematopoietic stem‐progenitor cell cultures is dependent on stage‐specific notch signaling. (A): Schematic diagram illustrating gamma secretase inhibitor (GSI) dosing scheme. (B): Photomicrograph of D15 cell cultures. Cultures were treated with GSI at D4–5 or D4 onward (D4+) and display dramatically reduced hematopoietic cell emergence. (C): Quantification of D15 suspension hematopoietic cells generated in control/GSI‐treated cultures; cell numbers were normalized to the control for each experimental set. Error bars = SD. Statistical significance determined by Student's t test analysis. (D): Analysis of D10 control and GSI‐treated cultures for CD34 and CD45 revealed intact endothelial cell formation (CD34 + CD45 ‐ ) but reduced hematopoietic cell formation (CD34 + CD45 + ) in GSI‐treated cultures. (E): Gene expression analyses of D10 control versus GSI‐treated cultures for a panel of hematopoietic and signaling genes of interest. DLL4, HEY1, GATA2, and AML1 are significantly downregulated in GSI‐treated cultures, as determined by Student's t test analysis. All expression levels normalized to beta actin housekeeping gene. Error bars = SD. (F): Globin gene expression analyses of erythroid cells generated from control and stage‐specific GSI‐treated cultures. Error bars = SD. Statistical significance determined by Student's t test analysis. Abbreviations: EHT, endothelial‐to‐hematopoietic transition; GATA2; GSI, gamma secretase inhibitor; HBA, hemoglobin comprised of alpha; HBB, hemoglobin comprised of beta; HBE, embryonic hemoglobin; HBG, hemoglobin comprised of gamma; HSPC, hematopoietic stem‐progenitor cell; iPSC, induced pluripotent stem cell; RUNX1; SCL.

Journal: Stem Cells (Dayton, Ohio)

Article Title: Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells

doi: 10.1002/stem.2822

Figure Lengend Snippet: Hematopoietic progenitor cell formation in induced pluripotent stem (iPS)–hematopoietic stem‐progenitor cell cultures is dependent on stage‐specific notch signaling. (A): Schematic diagram illustrating gamma secretase inhibitor (GSI) dosing scheme. (B): Photomicrograph of D15 cell cultures. Cultures were treated with GSI at D4–5 or D4 onward (D4+) and display dramatically reduced hematopoietic cell emergence. (C): Quantification of D15 suspension hematopoietic cells generated in control/GSI‐treated cultures; cell numbers were normalized to the control for each experimental set. Error bars = SD. Statistical significance determined by Student's t test analysis. (D): Analysis of D10 control and GSI‐treated cultures for CD34 and CD45 revealed intact endothelial cell formation (CD34 + CD45 ‐ ) but reduced hematopoietic cell formation (CD34 + CD45 + ) in GSI‐treated cultures. (E): Gene expression analyses of D10 control versus GSI‐treated cultures for a panel of hematopoietic and signaling genes of interest. DLL4, HEY1, GATA2, and AML1 are significantly downregulated in GSI‐treated cultures, as determined by Student's t test analysis. All expression levels normalized to beta actin housekeeping gene. Error bars = SD. (F): Globin gene expression analyses of erythroid cells generated from control and stage‐specific GSI‐treated cultures. Error bars = SD. Statistical significance determined by Student's t test analysis. Abbreviations: EHT, endothelial‐to‐hematopoietic transition; GATA2; GSI, gamma secretase inhibitor; HBA, hemoglobin comprised of alpha; HBB, hemoglobin comprised of beta; HBE, embryonic hemoglobin; HBG, hemoglobin comprised of gamma; HSPC, hematopoietic stem‐progenitor cell; iPSC, induced pluripotent stem cell; RUNX1; SCL.

Article Snippet: The following antibodies were used: HBB (16216‐1‐AP, Proteintech, Rosemont, IL), HBA (sc‐21756, Santa Cruz Biotechnology, Dallas, TX), and GAPDH (sc‐47724, Santa Cruz Biotechnology, Dallas, TX).

Techniques: Suspension, Generated, Control, Gene Expression, Expressing

The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). a UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of IL1 β and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.

Journal: Nature Communications

Article Title: Severe inflammation and lineage skewing are associated with poor engraftment of engineered hematopoietic stem cells in patients with sickle cell disease

doi: 10.1038/s41467-025-58321-4

Figure Lengend Snippet: The Cell-ID method was used to assess the statistical enrichment of individual-cell gene signatures vs. signaling pathway gene sets (such as Hallmark gene sets, MSigDB collections, v7.5.1) based on two-sided hypergeometric test p -values with Benjamini–Hochberg correction for the number of tested gene signatures. Enrichment scores were calculated as the -log10( p -value). a UMAP plots highlighting cells significantly matching the All HSC and MkP signatures, for each HD ( n = 3) and SCD patient ( n = 3) ( p < 0.05). Cells matching All HSC and MkP cell types are shown in black, and cells matching with only one cell type are shown in red (All HSC) and pink (MkP). The number of cells in each category and per patient, is depicted in the histogram on the right. b Boxplots of IL1 β and VWF mRNA expression in HSCs, HSC-enriched and MkP populations, in each HD ( n = 3) and SCD patient ( n = 3). c , d UMAP plots of TNFa and IFN gamma response pathway enrichment scores for each HD ( n = 3) and SCD patient ( n = 3), determined with Cell-ID. e , f Boxplots representing significant TNFa and IFN gamma enrichment scores ( p < 0.01) in HSCs for each HD ( n = 3) and SCD patient ( n = 3). Dotted lines represent the significant threshold -log 10 ( p -value = 0.01). g , h Boxplot representing CEBPB, MAFF, IFI44L , and MX1 mRNA expression in the HSCs populations in each HD ( n = 3) and SCD patient ( n = 3). In each boxplot, the edges of the box indicate the first and third quartiles and the center line indicates the data median. The whiskers denote 1.5× interquartile range, data beyond the end of the whiskers are called “outlying” points and are plotted individually.

Article Snippet: Briefly, RBC samples frozen in glycerol were thawed, fixed and permeabilized before being incubated with mouse monoclonal antibodies (Ab) against human γ-globin chain (IQ Products: IQP-363-INT-4), β S -globin chain (Rockland: 200-301-GS5) or βA-globin chain (Rockland: 200-301-GS4).

Techniques: Expressing